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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually described. Two more splice variants were noted but in depth nucleotide sequence info is just not still obtainable.

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Blocking peptides are peptides that bind exclusively to your focus on antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.

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This guidebook get more info outlines more possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...

Antibodies bound to the blocking peptide now not bind on the epitope around the focus on protein. By comparing the staining through the blocked antibody versus the antibody by yourself, one can see which staining is distinct.

Target Data Cyclin E belongs to the hugely conserved cyclin family, whose associates are characterised by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which lead to your temporal coordination of each mitotic occasion. Cyclin E kinds a posh with and capabilities as being a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is also degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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